Rat NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit

SKU: orb2998026

Description

Rat NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) Microsample ELISA Kit

Research Area

Signal Transduction

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.114 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B

Quick Database Links

UniProt

Q63369