Rat Melatonin (MT) ELISA Kit

SKU: orb1946814

Description

Rat Melatonin (MT) ELISA Kit

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Application Notes:
  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat MT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: serum, Brain dialysate
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

N-Acetyl-5-Methoxytryptamine

Standard curve for orb1946814 ELISA kit.

Quick Database Links

Gene Symbol

MT