Rat Melatonin (MT) ELISA Kit

• Catalog Number: orb1736603
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Concentration: 500 pg/mL
• Application notes: standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 2.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.78 pg/mL
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Alternative names: N-Acetyl-5-Methoxytryptamine
• Note: For research use only