Rat IgM (mu chain) Antibody Biotin Conjugated

SKU: orb347871

Description

Rat IgM (mu chain) antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Rat
• Application Notes:
  Anti-RAT IgM (mu chain) Antibody has been assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Rat IgM antibody is suitable for immunoassays including western blot and ELISA.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rat IgM whole molecule
• Purity: Anti-RAT IgM (mu chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Purified Anti-RAT IgM was Biotin Conjugated. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Rat IgM (mu chain) Antibody biotin Conjugated, Rabbit Anti-Rat IgM mu Antibody BAC Conjugation

Carbohydrate binding by BoSGBPMLG-A using a structurally diverse saccharide microarray. Heatmap comparing the binding patterns of BoSGBPMLG-A and selected control proteins. The microarray comprised soluble saccharides of different origins (fungal, bacterial, plant, and microalgal polysaccharides or glycoproteins) (see Table S1); the major backbone sequences are depicted at the bottom. The heatmap represents the relative binding intensities calculated as the percentage of the fluorescence signal intensity at 150 pg (0.5 mg/ml)/spot given by the saccharide probe most strongly bound by each protein (normalized as 100%). Results are detailed in Table S2. S. cerevisiae, Saccharomyces cerevisiae; N. oculata, Nanochloropsis oculata; P. palmata, Palmaria palmata; C. albicans, Candida albicans; M. tuberculosis, Mycobacterium tuberculosis; hMalectin, human malectin; TmCBM41, CBM41 of Thermotoga maritima; mDectin-1, murine dectin-1; CtCBM11, CBM11 of Clostridium thermocellum; ConA, concanavalin A; AAL, Aleuria aurantia lectin.