Rat IgG2a isotype control Fluorescein Antibody

• Catalog Number: orb343817
• Category: Proteins
• Description: Rat IgG2a isotype control Fluorescein Antibody
• Tested applications: DOT, ELISA, FC, FLISA, IF
• Isotype: IgG2a
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2000 - 1:20,000, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG2a confirmed by Dot Blot.
• Conjugation: FITC
• Source: Rat
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rat Isotype Control, Rat IgG2a FITC isotype, RAT I
• Note: For research use only
• Application notes: RAT IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
• Expiration Date: 12 months from date of receipt.