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Rat IgG2a isotype control Fluorescein Antibody
Catalog Number: orb343817
| Catalog Number | orb343817 |
|---|---|
| Category | Proteins |
| Description | Rat IgG2a isotype control Fluorescein Antibody |
| Isotype | IgG2a |
| Conjugation | FITC |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG2a confirmed by Dot Blot. |
| Tested applications | DOT, ELISA, FC |
| Dilution range | ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000 |
| Application notes | RAT IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user. |
| Source | Rat |
| Biological Origin | Rat |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive. |
| Alternative names | Rat Isotype Control, Rat IgG2a FITC isotype, RAT I Read more... |
| Note | For research use only |
Dot Blot results of Rat IgG2a Isotype Control Fluorescein Conjugated. Antigen: Rat IgG2a Isotype Control FITC Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT.
