Rat IgG F(c) antibody

Catalog Number: orb347849

Catalog Number	orb347849
Category	Antibodies
Description	Rat IgG F(c) antibody
Species/Host	Rabbit
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Reactivity	Rat
Isotype	IgG
Immunogen	Rat IgG F(c) fragment
Concentration	2.1 mg/mL
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/Appearance	Liquid (sterile filtered)
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab).
Conjugation	Unconjugated
Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Alternative names	Rabbit Anti Rat IgG F(c) Antibody, Rabbit Anti-Rat Read more...
Note	For research use only
Application notes	Anti-Rat IgG F(c) antibody is tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Expiration Date	12 months from date of receipt.

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain.
Source:	Rat

Sizes Available: 1 mg

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain.
Source:	Rat

Sizes Available: 1 mg

Conjugation:	Biotin
Purity:	This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, anti- Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain.
Source:	Rat

Sizes Available: 1 mg

Applications:	ELISA, IHC, SDS-PAGE, WB
Conjugation:	Unconjugated
Purity:	Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c). No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain.
Source:	Rat

Sizes Available: 1 mg