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Catalog Number | orb347800 |
---|---|
Category | Antibodies |
Description | Rat IgG F(c) antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Rat |
Isotype | IgG |
Immunogen | Rat IgG F(c) fragment |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:8,000 - 1:9,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Goat Anti Rat IgG F(c) Antibody, Goat Anti-Rat IgG Read more... |
Note | For research use only |
Application notes | Anti-Rat IgG F(c) antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, anti- Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
ELISA, IHC, SDS-PAGE, WB | |
Unconjugated | |
Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c). No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
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