Rat IgG Fc Antibody

• Catalog Number: orb347800
• Category: Antibodies
• Description: Rat IgG F(c) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Rat IgG F(c) fragment
• Antibody Type: Secondary Antibody
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:8,000 - 1:9,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab).
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Anti Rat IgG F(c) Antibody, Goat Anti-Rat IgG
• Note: For research use only
• Application notes: Anti-Rat IgG F(c) antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western blot of Goat Anti-Rat IgG F(c) secondary antibody. Lane 1: Rat Fc (p/n orb346338). Lane 2: None. Load: 50 ng per lane. Primary antibody: Rat IgG F(c) antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rat IgG F(c). Other band(s): None.