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Rat IgG F(ab')2 Antibody
Catalog Number: orb347850
| Catalog Number | orb347850 |
|---|---|
| Category | Antibodies |
| Description | Rat IgG F(ab')2 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Rat |
| Isotype | IgG |
| Immunogen | Rat IgG F(ab')2 fragment |
| Antibody Type | Secondary Antibody |
| Concentration | 7.3 mg/mL |
| Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c). |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | Rabbit Anti Rat IgG F(ab')2 Antibody, Rabbit Anti- Read more... |
| Note | For research use only |
| Application notes | Anti-Rat IgG F(ab')2 antibody has been tested by ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Anti-Rat IgG F(ab')2 (RABBIT) Antibody (p/n orb347850). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(ab')2 (RABBIT) Antibody (p/n orb347850) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
- Item 1 of 2
Rat IgG F(ab')2 Antibody [orb346339]
SDS-PAGE
Unconjugated
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Rat
2 mg - Item 1 of 1
Rat IgG F(ab')2 Agarose Antibody [orb346336]
IP
Agarose
This product is an purified Rat IgG isolated from normal Rat Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 of anti-Rat IgG.
Rat
5 mg - Item 1 of 1
Rat IgG F(ab')2 Fluorescein Antibody [orb346344]
FITC
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Rat
1 mg
