Rat Hyaluronic Acid (HA) ELISA Kit

SKU: orb2667421

Description

The Rat Hyaluronic Acid (HA) ELISA Kit is designed to quantify Hyaluronic Acid (HA) in rat serum, plasma and other biological fluids. The kit has a detection range of 15.63-1000 ng/mL and a sensitivity of 4.94 ng/mL.

Research Area

Cancer Biology, Gastroenterology & Hepatology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HA in the samples is then determined by comparing the OD of the samples to the standard curve.

  Read more...

Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 15.63-1000 ng/mL
• Sensitivity: 4.94 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hyaluronan; Hyaluronate

Quick Database Links

Gene Symbol

HA