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Catalog Number | orb707320 |
---|---|
Category | Proteins |
Description | Recombinant Rat GM-CSF Protein is produced by mammalian expression system and the target gene encoding Ala18-Lys144 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Rat |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CSF2 |
Entrez | 116630 |
UniProt ID | P48750 |
Protein Sequence | APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQKVDH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS pH 7.4. |
Alternative names | GMCSF protein, Granulocyte-macrophage colony-stimu Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
43 kDa (predicted); 43-47 and 36 kDa (reducing condition, due to glycosylation) |
95.40% | |
16.9 kDa (predicted); 23 and 20 kDa (reducing conditions) |
> 97% by SDS-PAGE. | |
Recombinant Rat GM-CSF/CSF2 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala18-Lys144) of rat GM-CSF/CSF2 (Accession #NP_446304.1.) fused with a 6��His Tag at the N-terminus. |
> (32.5+65.6)%, determined by SDS-PAGE | |
This protein contains the rat CSF2 (P48750) (Ala18-Lys144) was expressed with a polyhistidine tag at the N-terminus and expressed from HEK293 Cells. |