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| Catalog Number | orb1784730 |
|---|---|
| Category | Proteins |
| Description | Recombinant Rat GM-CSF |
| Tag | C-terminal His Tag |
| Reactivity | Rat |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
| Purity | Greater than 95% as determined by SDS-PAGE |
| UniProt ID | P48750 |
| MW | 20-23 kDa |
| Tested applications | ELISA, WB |
| Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
| Source | HEK293 cells |
| Expression Region | 18-144 |
| Storage | -20°C for 12 months as lyophilized; 2-8°C for 1 month under sterile conditions after reconstitution |
| Alternative names | Colony stimulating factor, CSF, CSF2, GM CSF, GMCS Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 15.6 KDa. Observed: 17-25 KDa, reducing conditions |
> 97% by SDS-PAGE. | |
Recombinant Rat GM-CSF/CSF2 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala18-Lys144) of rat GM-CSF/CSF2 (Accession #NP_446304.1.) fused with a 6��His Tag at the N-terminus. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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