Rat Coagulation Factor II (F2) ELISA Kit

SKU: orb775381

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor II(F2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: Serum
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 ng/mL
• Sensitivity: 3.4 ng/mL
• Concentration: 500 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

FII ELISA Kit, TM ELISA Kit, PT ELISA Kit, Thrombin ELISA Kit, Prothrombin ELISA Kit, Pro-Thrombin ELISA Kit, Activation peptide fragment 1 ELISA Kit, Activation peptide fragment 2 ELISA Kit

Quick Database Links

UniProt

P18292