Rat Protease Activated Receptor 2 (PAR2) ELISA Kit

SKU: orb775502

Description

The Rat Protease Activated Receptor 2 (PAR2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Protease Activated Receptor 2 (PAR2) in rat tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.053 ng/mL.

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAR2 in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

F2RL1 ELISA Kit, F2-RL1 ELISA Kit, GPR11 ELISA Kit, PAR2 ELISA Kit, Coagulation Factor II(thrombin)receptor-Like 1 ELISA Kit, G-protein coupled receptor 11 ELISA Kit, Thrombin receptor-like 1 ELISA Kit

Quick Database Links

UniProt

Q63645