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Catalog Number | orb1473264 |
---|---|
Category | Proteins |
Description | Recombinant Rat BDNF Protein |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Rat |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | BDNF |
Entrez | 24225 |
UniProt ID | P23363 |
Protein Sequence | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
Alternative names | Brain-derived neurotrophic factor; BDNF; Abrineuri Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
16.3 kDa | |
E.coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
13 KDa | |
Mammalian |
> 99.9% | |
14 KDa (reducing condition) |
> 97% by SDS-PAGE and HPLC analyses. | |
28 kDa, a noncovalently linked homodimer of two 14.0 kDa polypeptide monomers (260 total amino acid residues). | |
Escherichia coli. |