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Rat anti Heparan Sulphate Proteoglycan

Catalog Number: orb98335

DispatchUsually dispatched within 5-10 working days
$ 570.00
Catalog Numberorb98335
CategoryAntibodies
DescriptionRat monoclonal to Heparan Sulphate Proteoglycan
Species/HostRat
ClonalityMonoclonal
Clone NumberA7L6
Tested applicationsICC, IHC-Fr, IHC-P, IP, WB
ReactivityBovine, Fish, Human, Mouse, Rat
IsotypeIgG2a
Antibody TypePrimary Antibody
Form/AppearanceThe vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.
ConjugationUnconjugated
Hazard InformationThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Biorbyt accepts no liability for any inaccuracies or omissions in this information.
UniProt IDP98160
SourceA7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesThe vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.
NoteFor research use only
Expiration Date12 months from date of receipt.
Rat anti Heparan Sulphate Proteoglycan

Immunofluorescence analysis of mouse with Hurler disease using Heparan Sulphate Proteoglycan antibody.

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Rat anti Heparan Sulphate Proteoglycan (orb98335)

We stained fresh frozen retinal/eye tissues. We tested both 1:100 and 1:200, and both worked - 1:100 was a bit brighter qualitatively, but we did not measure it. We tested both no fix and 4% para. Both work fine, although 1:200 with 4% para fix was noticeably dimmer.

- Linda McLoon/University of Minnesota

Applications

IHC

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