RASSF2 Antibody

• Catalog Number: orb1925897
• Category: Antibodies
• Description: RASSF2 Antibody
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a,κ
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: P50749
• MW: 37790 Da
• Tested applications: FC, WB
• Dilution range: FC - 1:25, WB - 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CENP-34 {ECO:0000303|PubMed:20813266}, K
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

Anti-RASSF2 Antibody at 1:2000 dilution + Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Raji cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.