RASGRP2 Antibody (N-term)

• Catalog Number: orb1937682
• Category: Antibodies
• Description: RASGRP2 Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q7LDG7
• MW: 69248 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CDC25L, MCG7
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only

Anti-RASGRP2 Antibody (N-term) at 1:1000 dilution + human spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

RASGRP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

RASGRP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).