RASGRP1 Antibody (Center)

SKU: orb1930325

Description

RASGRP1 Antibody (Center)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 90402 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

RASGRP

RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

RASGRP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

RASGRP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the RASGRP1 antibody detected the RASGRP1 protein (arrow).

All lanes: Anti-RASGRP1 Antibody (Center) 1:1000 dilution. Lane 1: A549 whole cel lysate. Lane 2: SW480 whole cel lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 100kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

O95267