RASGRF1 Antibody

• Catalog Number: orb1256692
• Category: Antibodies
• Description: RASGRF1 Antibody
• Target: RASGRF1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1).
• UniProt ID: Q13972
• MW: Observed: 145kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ras-specific guanine nucleotide-releasing factor 1
• Research Area: Cell Biology, Signal Transduction
• Note: For research use only

Western blot analysis of extracts of mouse brain, using RASGRF1 antibody (orb1256692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody (orb1256692) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using RASGRF1 antibody (orb1256692). Blue: DAPI for nuclear staining.