RANTES Recombinant Rabbit Monoclonal Antibody

SKU: orb704344

Description

RANTES Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B8C3
• Immunogen: KLH conjugated synthetic peptide derived from human RANTES
• Target: CCL5
• Molecular Weight: 10 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D17S136E; RANTES; SCYA5; SIS-delta; SISd; TCP228; eoCP; CCL5_HUMAN; CCL5; Eosinophil chemotactic cytokine; Small-inducible cytokine A5; T cell-specific protein P228 (TCP228); T-cell-specific protein RANTES;

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (RANTES) monoclonal Antibody, Unconjugated (orb704344) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (RANTES) monoclonal Antibody, Unconjugated (orb704344) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human breast cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RANTES) Monoclonal Antibody, Unconjugated (orb704344) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung caner), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RANTES) Monoclonal Antibody, Unconjugated (orb704344) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RANTES) Monoclonal Antibody, Unconjugated (orb704344) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: mouse thymus tissue lysate, Primary: Anti-RANTES (orb704344) at 1:1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:20000 dilution, Predicted band size: 7.4/10 kD, Observed band size: 15 kD.

Quick Database Links

Gene Symbol

CCL5

UniProt

P13501