RAMP3 Antibody

SKU: orb1536901

Description

RAMP3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC-P, WB
• Dilution Range: ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: Western blot analysis of RAMP3 expression in MCF7 (A), mouse brain (B), mouse heart (C), rat brain (D), rat lung (E) whole cell lysates. Immunofluorescent analysis of RAMP3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of human RAMP3
• Target: RAMP3
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAMP3

Quick Database Links

Gene Symbol

RAMP3