RAMP3 Antibody

• Catalog Number: orb1536901
• Category: Antibodies
• Description: RAMP3 Antibody
• Target: RAMP3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.. Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Immunoaffinity chromatography
• Immunogen: Recombinant full length protein of human RAMP3
• Tested applications: ICC, IF, IHC-P, WB
• Dilution range: ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)
• Application notes: Further information: Western blot analysis of RAMP3 expression in MCF7 (A), mouse brain (B), mouse heart (C), rat brain (D), rat lung (E) whole cell lysates. Immunofluorescent analysis of RAMP3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RAMP3
• Note: For research use only