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Item 1 of 6
Item 1 of 6
RalA Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb2563052
| Catalog Number | orb2563052 |
|---|---|
| Category | Antibodies |
| Description | RalA Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 1E16 |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human RalA |
| Antibody Type | Recombinant Antibody |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 24 kDa |
| Target | RALA |
| UniProt ID | P11234 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Ral a; Ral A protein; RAL; RALA; RALA_HUMAN; RAS l Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
25 ug total protein per lane of various lysates probed with RalA monoclonal antibody, unconjugated (orb2563052) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with RalA Monoclonal Antibody, Unconjugated (orb2563052) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with RalA Monoclonal Antibody, Unconjugated (orb2563052) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with RalA Monoclonal Antibody, Unconjugated (orb2563052) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with RalA Monoclonal Antibody, Unconjugated (orb2563052) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with RalA Monoclonal Antibody, Unconjugated (orb2563052) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
