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Raf1 Rabbit pAb

SKU: orb1563431

Description

Raf1 Rabbit pAb

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, ICC, IF, IHC-Fr, IHC-P, WB
Dilution RangeWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human C-RAF.
TargetRAF1
Molecular WeightCalculated MW: 73 kDa; Observed MW: 73 kDa
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesLiquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

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Raf1 Rabbit pAb

Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.

Raf1 Rabbit pAb

Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.

Raf1 Rabbit pAb

Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Raf1 Rabbit pAb

Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody (red), and DAPI (blue).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Raf1 Rabbit pAb (orb1563431)

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Available Sizes

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20 μl
$ 120.00
50 μl
$ 210.00
100 μl
$ 350.00
DispatchUsually dispatched within 5-10 working days
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