RAF1 Antibody

• Catalog Number: orb97435
• Category: Antibodies
• Description: Mouse monoclonal antibody to RAF1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4G4E5
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human RAF1 expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 73kDa
• Target: RAF1
• Entrez: 5894
• UniProt ID: P04049
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: NS5; CRAF; Raf-1; c-Raf
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of RAF1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded bladder cancer tissues using RAF1 antibody

Flow cytometric analysis of MCF-7 cells using RAF1 antibody

Western blot analysis of human (AA: 198-407) recombinant protein using RAF1 antibody

Western blot analysis of HeLa (Lane1), A431 (Lane2), HepG (Lane3), and SW620 (Lane4)cell lysate using RAF1 antibody