RAF1 Antibody

SKU: orb97435

Description

Mouse monoclonal antibody to RAF1

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 4G4E5
• Immunogen: Purified recombinant fragment of human RAF1 expressed in E. Coli.
• Target: RAF1
• Molecular Weight: 73kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Alternative Names

NS5; CRAF; Raf-1; c-Raf

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of RAF1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded bladder cancer tissues using RAF1 antibody

Flow cytometric analysis of MCF-7 cells using RAF1 antibody

Western blot analysis of human (AA: 198-407) recombinant protein using RAF1 antibody

Western blot analysis of HeLa (Lane1), A431 (Lane2), HepG (Lane3), and SW620 (Lane4)cell lysate using RAF1 antibody

Quick Database Links

Gene Symbol

RAF1

UniProt

P04049

NCBI Gene ID

5894