Raf-1 rabbit pAb Antibody

SKU: orb766189

Description

Rabbit polyclonal antibody to Raf-1

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:11-60
• Molecular Weight: 73
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RAF1 antibody, anti-RAF antibody, anti-RAF proto-oncogene serine/threonine-protein kinase antibody, anti-Proto-oncogene c-RAF antibody, anti-cRaf antibody, anti-Raf-1 antibody

Western blot analysis in MCF-7 cells transfected with siRaf-1. Cancer Letters 355 (2014) 288–296.

Western Blot analysis of various cells using Raf-1 Polyclonal Antibody diluted at 1:2000.

The picture was kindly provided by our customer.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using C-RAF Antibody. The picture on the right is blocked with the synthesized peptide.