A-Raf (phospho Tyr302) rabbit pAb Antibody

SKU: orb768828

Description

Rabbit polyclonal antibody to A-Raf (phospho-Tyr302)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. AA range:276-325
• Molecular Weight: 68
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-ARAF antibody, anti-ARAF1 antibody, anti-PKS antibody, anti-PKS2 antibody, anti-Serine/threonine-protein kinase A-Raf antibody, anti-Proto-oncogene A-Raf antibody, anti-Proto-oncogene A-Raf-1 antibody, anti-Proto-oncogene Pks antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using A-RAF (Phospho-Tyr302) Antibody.

Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide.