A-Raf rabbit pAb

• Catalog Number: orb764568
• Category: Antibodies
• Description: Rabbit polyclonal antibody to A-Raf
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:276-325
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 68
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-ARAF antibody, anti-ARAF1 antibody, anti-PKS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis in MCF-7 cells transfected with siA-Raf. Cancer Letters 355 (2014) 288–296.

Western Blot analysis of various cells using A-Raf Polyclonal Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using A-RAF Antibody. The lane on the right is blocked with the synthesized peptide.