RAE1 Antibody

SKU: orb1247041

Description

RAE1 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NAAEELKPRNKK
• Target: RAE1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

RAE1, RAE1 RNA export 1 homolog (S. pombe) , HGNC:9828, FLJ30608, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1, RAE1 (RNA export 1, S.pombe) homolog, homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer), mRNA export protein, mRNA-associated protein MRNP 41, mRNA-binding protein, 41-kD, migration-inducing gene 14, RP4-800J21.2, MGC117333, MGC126076, MGC126077, OTTHUMP00000166110

orb1247041 (0.1 ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247041 (3.8 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247041 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RAE1

UniProt

P78406

RefSeq (Protein):NP_001015885.1, NP_003601.1