RAE1 Antibody

• Catalog Number: orb1247041
• Category: Antibodies
• Description: RAE1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NAAEELKPRNKK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RAE1
• UniProt ID: P78406
• NCBI: NP_003601.1, NP_001015885.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RAE1, RAE1 RNA export 1 homolog (S. pombe) , HGNC:
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates cell lines HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0kDa according to NP_003601.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows staining of neuronal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Heart. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247041 (0.1 ug/ml) staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247041 (3.8 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247041 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.