RAD9A Antibody

• Catalog Number: orb1258404
• Category: Antibodies
• Description: RAD9A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 27kDa
• Target: RAD9A
• UniProt ID: Q99638
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: RAD9A, RAD9 homolog A (S. pombe), RAD9, RAD9 (S. p
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse testis, using RAD9A antibody (orb1258404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (orb1258404) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody (orb1258404) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (orb1258404). Blue: DAPI for nuclear staining.