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RAD52 Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5E11E7 |
| Immunogen | Purified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. Coli. |
| Target | RAD52 |
| Molecular Weight | 46.2kDa |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
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Immunofluorescence analysis of HeLa cells using RAD52 antibody

Western blot analysis of human recombinant protein using RAD52 antibody

Western blot analysis of HEK293 using RAD52 antibody

Western blot analysis of HepG2 (Lane1), MCF-7 (Lane2), MCF-7 (Lane3), and C6 (Lane4) cell lysate using RAD52 antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of MCF7 cells using RAD52 antibody
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Protocol Information
RAD52 Antibody (orb333951)
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