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Catalog Number | orb333951 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to RAD52 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 5E11E7 |
Tested applications | FC, ICC, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. Coli. |
Antibody Type | Primary Antibody |
Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
Dilution range | WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
Conjugation | Unconjugated |
MW | 46.2kDa |
Target | RAD52 |
Entrez | 5893 |
UniProt ID | P43351 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of HeLa cells using RAD52 antibody
Western blot analysis of human recombinant protein using RAD52 antibody
Western blot analysis of HEK293 using RAD52 antibody
Western blot analysis of HepG2 (Lane1), MCF-7 (Lane2), MCF-7 (Lane3), and C6 (Lane4) cell lysate using RAD52 antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of MCF7 cells using RAD52 antibody