RAC2 Antibody

SKU: orb1247906

Description

RAC2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of Human Thymus and Tonsil and of cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 0.1-0.3µg/mL. Preliminary testing was unsuccessful on Rat and Mouse Spleen, and cell line NIH-3T3 for this particular batch.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: PQPTRQQKRACSLL
• Target: RAC2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAC2, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2), EN-7, Ras-related C3 botulinum toxin substrate 2, rho family, small GTP binding protein Rac2, Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2), Gx, HSPC022, small G protein

orb1247906 (0.1 ug/ml) staining of Human Thymus (A) and Tonsil (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247906 (0.3 ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.

orb1247906 (0.3 ug/ml) staining of MOLT4 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

RAC2

UniProt

P15153

RefSeq (Protein):NP_002863.1