RAC2 Antibody

• Catalog Number: orb1247906
• Category: Antibodies
• Description: RAC2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: PQPTRQQKRACSLL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of Human Thymus and Tonsil and of cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on Rat and Mouse Spleen, and cell line NIH-3T3 for this particular batch.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RAC2
• UniProt ID: P15153
• NCBI: NP_002863.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RAC2, ras-related C3 botulinum toxin substrate 2 (
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of Human Thymus and Tonsil and of cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on Rat and Mouse Spleen, and cell line NIH-3T3 for this particular batch.
• Expiration Date: 12 months from date of receipt.

orb1247906 (0.1 ug/ml) staining of Human Thymus (A) and Tonsil (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247906 (0.3 ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.

orb1247906 (0.3 ug/ml) staining of MOLT4 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.