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Catalog Number | orb766174 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Rac GAP1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Monkey, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human GTPase Activating Protein. AA range:353-402 |
MW | 72 |
Tested applications | ELISA, IF, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-RACGAP1 antibody, anti-KIAA1478 antibody, ant Read more... |
Note | For research use only |
Western Blot analysis of various cells using Rac GAP1 Polyclonal Antibody.
Immunofluorescence analysis of HeLa cells, using GTPase Activating Protein Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, using GTPase Activating Protein Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
ELISA, IF, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
IRDye800 |
IF | |
Bovine, Canine, Human, Mouse | |
Rat | |
Rabbit | |
Polyclonal | |
PerCP/Cy7 |
IF | |
Bovine, Canine, Human, Mouse | |
Rat | |
Rabbit | |
Polyclonal | |
Pacific Blue |