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ZFYVE20 Antibody

SKU: orb1247046

Description

ZFYVE20 Antibody

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-ELKHTLAKQKGGTD
TargetZFYVE20
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

ZFYVE20, Rabenosyn 5, FYVE-finger-containing Rab5 effector protein rabenosyn-5, zinc finger, FYVE domain containing 20, zinc finger, FYVE domain containing 20, FLJ34993, MGC126210, Rabenosyn-5

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ZFYVE20 Antibody

orb1247046 (0.3 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ZFYVE20 Antibody (orb1247046)

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Available Sizes

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0.1 mg
$ 880.00
DispatchUsually dispatched within 5-10 working days
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