Rabbit polyclonal antibodies to human IFN-gamma

• Catalog Number: orb2284549
• Category: Antibodies
• Description: Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon
• Target: Interferon-gamma
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG/IgM
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: Batch dependent within range 2.0-2.5 mg/mL
• Buffer/Preservatives: Glycine pH 7.2
• Purification: Immunogen affinity column
• Immunogen: Recombinant human IFN-Gamma (P01579)
• UniProt ID: PO1579
• MW: 17 kDa
• Tested applications: ELISA, WB
• Dilution range: Western blot: 0.1-1.0 µg/ml
• Cross Reactivity: May cross-react with other species
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: NP_000610.2
• Expiration Date: 12 months from date of receipt.

Western blot demonstrating our polyclonal detecting recombinant human Interferon-Gamma (24-166 of P01579) at 1 in 2000 dilution. Lane 1: Marker; Lane 2: IFN-γ (1ng); Lane 3: IFN-γ (5ng); Lane 4: IFN-γ (10ng); Lane 5: IFN-γ (50ng); Lane 6: Blank; Lane 7: IFN-γ (100ng); Lane 8: Blank; Lane 9: IFN-γ (200ng); Lane 10: Blank.

Recombinant Human Interferon gamma detected in a competition ELISA with monoclonal anti-Interferon gamma as the coating antibody, our polyclonal competes for binding to interferon gamma with a biotinylated polyclonal anti-human interferon gamma and detection is with Streptavidin-HRP.