Rabbit polyclonal antibodies to Beta-2-Microglobulin

SKU: orb2284544

Description

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: Western blot: 0.1-1.0 µg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG/IgM
• Immunogen: B2MG_Human 21-119
• Target: Beta-2-Microglobulin (B2M)
• Molecular Weight: 11 731 Da
• Purification: Immunogen affinity column
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Glycine pH 7.2
• Concentration: Batch dependent within range 2.0-2.5 mg/mL
• Disclaimer: For research use only

Western blot demonstrating our polyclonal detecting recombinant human Beta-2 Microglobulin (21-119 of P61769) at 1 in 2000 dilution. Lane 1: Marker; Lane 2: β2M (1ng); Lane 3: β2M (5ng); Lane 4: β2M (10ng); Lane 5: β2M (50ng).

ELISA Plates were coated with 0.6 µg/mL human recombinant Beta-2-microglobulin, rabbit anti-Beta-2-microglobulin was added at different concentrations and detected with an anti-rabbit HRP.

Quick Database Links

Gene Symbol

Beta-2-Microglobulin (B2M)

UniProt

P61769

RefSeq (Protein):NP_004039.1