Rabbit polyclonal antibodies to NO Synthase

SKU: orb2284542

Description

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: Western blot: 1.0 -5.0 µg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG/IgM
• Immunogen: Peptide conjugated to KLH (RHLRGAVPWAFDPPGPDTPGP)
• Target: Endothelial Nitric Oxide Synthase
• Molecular Weight: 133 287 Da
• Purification: Immunogen affinity column
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Glycine pH 7.2
• Concentration: Batch dependent within range 0.8-1.2 mg/mL
• Disclaimer: For research use only

Western blot demonstrating our polyclonal detecting recombinant human eNOS (P29474) at 1 in 500 dilution. Lane 1: Marker; Lane 2: eNOS (200ng); Lane 3: eNOS (300ng); Lane 4: eNOS (400ng).

ELISA Plates were coated with 1 µg/mL recombinant human eNOS, our polyclonal rabbit anti eNOS was added at different concentrations and detected with an anti-rabbit HRP.

Quick Database Links

Gene Symbol

Endothelial Nitric Oxide Synthase

UniProt

P29474

RefSeq (Protein):NP_000594.2