Rabbit IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

• Catalog Number: orb347655
• Category: Antibodies
• Description: Rabbit IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: AP
• Reactivity: Rabbit
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Purity: Rabbit IgG (H&L) Alkaline Phosphatase Pre-Adsorbed Conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Immunogen: Rabbit IgG whole molecule
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.
• Antibody Type: Secondary Antibody
• Storage: Store secondary antibody at 4° C before opening. DO NOT FREEZE. Alkaline Phosphatase conjugated antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: goat anti-Rabbit IgG Antibody Alkaline Phosphatase
• Note: For research use only

References

Tabata, Shinpei et al. The influence of isoflavone for denervation-induced muscle atrophy Eur J Nutr, 58, 291-300 (2019)

PubMed: 29236164

Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n orb347649). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n orb347649) 1:1000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.