Rabbit IgG (H&L) Antibody Pre-Adsorbed

• Catalog Number: orb347716
• Category: Antibodies
• Description: RABBIT IgG (H&L) antibody
• Clonality: Polyclonal
• Species/Host: Guinea pig
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rabbit
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.20 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins.
• Immunogen: Rabbit IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:15,500 - 1:17,500, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Rabbit IgG antibody has been tested by ELISA, SDS-PAGE, and Western Blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Guinea Pig Anti-Rabbit IgG Antibody, Guinea Pig An
• Note: For research use only

ELISA Results of Purified Guinea Pig Anti-Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, Mouse IgG). Each well was coated in duplicate with 10 µg of Rabbit IgG (p/n orb2652747) [Red Line], Human IgG (p/n orb346219) [Green Line], Goat IgG (p/n orb2652767) [Blue Line], Mouse IgG (p/n orb2652749) [Purple Line]. The working dilution is 1:17000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Guinea Pig mx10 HRP conjugated (p/n orb347053) and TMB substrate (p/n orb348651).

Western Blot of Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347716). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347716) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Pig IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347053) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.