Rabbit IgG (H&L) Antibody

• Catalog Number: orb346681
• Category: Antibodies
• Description: Rabbit IgG (H&L) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat.
• Antibody Type: Secondary Antibody
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit IgG, Ra
• Note: For research use only
• Application notes: Anti-Rabbit IgG whole molecule has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Goat Anti-Rabbit IgG Antibody. Each well was coated in duplicate with 1.0 µg of Rabbit IgG (p/n orb2652747). The working dilution is 10000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Donkey Anti-Goat IgG HRP conjugated (p/n orb347032), and TMB substrate (p/n orb348651).