Rabbit IgG (5B2) Mouse mAb

SKU: orb1563302

Description

Rabbit IgG (5B2) Mouse mAb

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB: 1/500-1/1000 ELISA: 1/10000
• Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5B2-F9-D6
• Immunogen: Rabbit IgG
• Target: Rabbit IgG
• Molecular Weight: Calculated MW: 55,25 kDa; Observed MW: 55,25 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Concentration: 5.85 mg/ml
• Disclaimer: For research use only

Alternative Names

Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS.

Western blot analysis of MEK2 in THP-1, Jurkat, Raji, 3T3 and Hela lysates using MEK2 antibody, then incubated with orb1563302 (Rabbit IgG mAb).Anti-mouse IgG, HRPlinked antibody was used for detecting.

Western blot analysis of Rabbit IgG (5B2) in decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using IgG (HeavyChain Specific) antibody.

Immunoprecipitation analysis of Rabbit IgG (5B2) in CHO-K1 lysates transfected with HAtagged NGAL using HATag antibody (lane 2). Lane 1 contains rabbit IgG IP control lysates. All lanes are probed with HATag antibody as the primary antibody. Panel A incubated with secondary antibodies anti-rabbit whole IgG, HRPlinked antibody for detecting.Panel B incubated with anti-rabbit IgG (HeavyChain Specific) mAb and the Anti-mouse whole IgG, HRPlinked antibody was used for detecting.

Quick Database Links

Gene Symbol

Rabbit IgG