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Rabbit anti-ZONAB Antibody
Catalog Number: orb1525512
| Catalog Number | orb1525512 |
|---|---|
| Category | Antibodies |
| Description | ZONAB Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 250 and 300 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | ZONAB |
| UniProt ID | P16989 |
| NCBI | NP_003642.3 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | DNA-binding protein A; cold shock domain-containin Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human ZONAB by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, HeLa, Hep-G2, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZONAB antibody (orb1525512) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human ZONAB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZONAB antibody (orb1525512) used for IP at 6 µg per reaction. ZONAB was also immunoprecipitated by a previous lot of this antibody (orb1525512) and a second antibody against a different epitope of ZONAB.
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