Rabbit anti-vPARP Antibody

SKU: orb1527494

Description

vPARP Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1674 and C-terminus
• Target: vPARP

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

VPARP; PH5P; poly [ADP-ribose] polymerase 4; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; von Willebrand factor A domain containing 5C; PARP-4; VWA5C; VAULT3; ADP-ribosyltransferase diphtheria toxin-like 4; PARP-related; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; PARP-related/IalphaI-related H5/proline-rich; ADPRTL1; ARTD4; H5 proline-rich; I-alpha-I-related; p193; PARPL; 193 kDa vault protein

Detection of human vPARP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-vPARP antibody orb1527494 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human vPARP by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-vPARP (Cat. No. orb1527494) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

vPARP

UniProt

Q9UKK3

RefSeq (Protein):NP_006428.2