Rabbit VMA21 Antibody

SKU: orb1520593

Description

VMA21 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 20 µl/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: VMA21
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MEAX; myopathy with excessive autophagy protein; vacuolar ATPase assembly integral membrane protein VMA21; VMA21 vacuolar H+-ATPase homolog; XMEA

Detection of human and mouse VMA21 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VMA21 antibody (orb1520593) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human VMA21 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VMA21 antibody (orb1520593) used for IP at 2 µg per reaction.

Quick Database Links

Gene Symbol

VMA21

UniProt

Q3ZAQ7

RefSeq (Protein):NP_001017980.1