Rabbit Valyl-tRNA Synthetase/VARS Antibody

SKU: orb1522351

Description

Valyl-tRNA Synthetase/VARS Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1214 and C-terminus
• Target: Valyl-tRNA Synthetase/VARS
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

G7A; NDMSCA; protein G7a; valine tRNA ligase 1, cytoplasmic; valine--tRNA ligase; valRS; Valyl-tRNA synthetase; valyl-tRNA synthetase 2; VARS1; VARS2

Detection of human and mouse Valyl-tRNA Synthetase/VARS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Valyl-tRNA Synthetase/VARS antibody orb1522351 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Valyl-tRNA Synthetase/VARS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Valyl-tRNA Synthetase/VARS antibody orb1522351 used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

Valyl-tRNA Synthetase/VARS

UniProt

P26640

RefSeq (Protein):NP_006286.1