Rabbit anti-USF2 Antibody

• Catalog Number: orb1524639
• Category: Antibodies
• Description: USF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: USF2
• UniProt ID: Q15853
• NCBI: NP_003358.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: major late transcription factor 2; bHLHb12; upstre
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human USF2 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , and Jurkat (J; 50 µg) cells. Antibody: Affinity purified rabbit anti-USF2 antibody orb1524639 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human USF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USF2 antibody orb1524639 used ffor IP at 6 µg/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies, which recognize downstream epitopes. For blotting immunoprecipitated USF2, orb1524639 was used at 1.0 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.

Detection of human USF2 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-USF2 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse USF2 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-USF2 (Cat. No. orb1524639) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB