Rabbit anti-UBQLN4/CIP75/Ubiquilin 4 Recombinant Monoclonal Antibody

• Catalog Number: orb1519632
• Category: Antibodies
• Description: UBQLN4/CIP75/Ubiquilin 4 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR145J
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Residues 2-601 (full length)
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1,000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: UBQLN4/CIP75/Ubiquilin 4
• UniProt ID: Q9NRR5
• NCBI: NP_064516.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: ataxin-1 ubiquitin-like-interacting protein A1U; u
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human UBQLN4 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit anti-UBQLN4 recombinant monoclonal antibody (orb1519632) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human UBQLN4/CIP75/Ubiquilin 4 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody (orb1519632) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.