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Rabbit TRAX Antibody
Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:5,000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 240 and C-terminus |
| Target | TRAX |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of human TRAX by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAX antibody orb1522817 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human TRAX by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TRAX (Cat. No. orb1522817) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human TRAX by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-TRAX (Cat. No. orb1522817) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005990.1 |
|---|
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Protocol Information
Rabbit TRAX Antibody (orb1522817)
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