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Rabbit Topo II Beta Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1576 and C-terminus |
| Target | Topo II Beta |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human and mouse Topo II Beta by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-Topo II Beta antibody orb1529340 at 0.04 µg/ml.

Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of human Topo II Beta by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001059.2 |
|---|
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Protocol Information
Rabbit Topo II Beta Antibody (orb1529340)
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