Rabbit Topo II Alpha Antibody

SKU: orb1530753

Description

Topo II Alpha Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:10,000 - 1:30,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1481 and C-terminus
• Target: Topo II Alpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; topoisomerase (DNA) II alpha 170kDa; TP2A

Detection of human TOPO-II-Alpha by western blot.Samples: Whole cell lysate from Jurkat, HeLa, and HEK293T cells. Antibody: Affinity purified rabbit anti-TOPO-II-Alpha antibody orb1530753 at 0.04 µg/ml.

Detection of human Topo II Alpha by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II alpha used at a dilution of 1:1, 000 (1µg/ml).

Detection of human TOPO-II-Alpha by western blot of immunoprecipitates.Samples: Whole cell lysate from Jurkat cells. Antibodies: Affinity purified rabbit anti-TOPO-II-Alpha antibody.

Quick Database Links

Gene Symbol

Topo II Alpha

UniProt

P11388

RefSeq (Protein):NP_001058.2