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Description
Images & Validation
−| Tested Applications | IP |
|---|---|
| Dilution Range | WB - Not recommended; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 100 and 150 |
| Target | TMF1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human TMF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-TMF1 antibody orb1525220 used for IP at 6 µg/mg lysate. TMF1 was also immunoprecipitated by rabbit anti-TMF1 antibodies, which recognize other epitopes. For blotting immunoprecipitated TMF1, orb1525218 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time 10 seconds.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_009045.2 |
|---|
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Rabbit TMF1 Antibody (orb1525220)
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